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2021-6-4 · The five highest rated assays for each target sequence were then chosen for testing. The length of the amplicons was kept between 100 and 250 bp for optimal amplifiion efficiency. The specificity of the primers was confirmed by qPCR (20 ng of cDNA used in each qPCR reaction), and product size was observed by electrophoresis on 2% agarose gels.
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Comparison of DNA yields using the Wizard® SV and SV 96 Genomic DNA Purifiion Systems. Average yield of genomic DNA in micrograms purified from 20mg mouse tail clippings. The average A 260 /A 280 ratios areSV 96, 1.7 ± 0.08; SV vacuum method, 1.7 ± 0.14; SV spin method, 1.7 ± 0.14.
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-12-6 · Outcomes measures, evaluated at bassline and weeks 4, 8 and 12, included two clinician rated scales, Ohio State Autism Rating Scale (OARS-4), and Clinical Global Improvement Scale and several
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2021-1-6 · Clothing is one of the primary human needs, and the demand is met by the global production of thousands of tons of textile fibers, fabrics and garments every day. Polyester clothing manufactured from oil-based polyethylene terephthalate (PET) is the market leader. Conventional PET creates pollution along its entire value chain—during the production, use and end-of-life phases—and also
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-2-26 · They''re the thievers of food, the pinchers of chips, the scourge of the seaside skies. Now scientists have revealed what we''ve always suspected — seagulls do prefer to eat food that has been
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